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Testing for PNH
Testing for PNH
High-Sensitivity Labs for PNH Diagnostic Laboratory Services
Flow cytometry, performed on peripheral blood, is the most sensitive and informative assay for PNH diagnosis.
Clear reporting is critical for appropriate clinical decisions.
When ordering a PNH flow test, request1:
- Clone size for each cell lineage (ie, granulocytes, monocytes, and erythrocytes)
- Proportion of type I, II, and III cells in cases in which PNH clones are detected
- Sensitivity level used (0.01% sensitivity is ideal)
- All previous flow results in order to monitor clonal expansion
With PNH, the use of high-quality pathology services that emphasize best practices and employ staff experienced in hematopathology are encouraged.
Labs should perform PNH flow cytometry, obtained from peripheral blood, on granulocytes and at least 1 additional cell line using >1 reagent.1,2
- PNH consensus guidelines recommend routine (1% PNH cell threshold) or high-sensitivity (0.01% PNH cell threshold) flow cytometry testing on patients at higher risk for PNH1
- Granulocytes should be evaluated with CD16, CD24, CD66b, or the FLAER assay1
- Flow cytometry is noninvasive and relatively inexpensive
Consider the following high-sensitivity labs for PNH diagnostic laboratory services:
US Labs & Lab Corp
www.labcorp.com
1-800-874-8532 / 1-800-710-1800
DAHL– Chase Diagnostic Services
www.dahlchase.net
1-877-PNH-FLOW (1-877-764-3569)
Hematogenix Laboratory Services
www.Hematogenix.com
(1-888)Hemato1 (1-708-444-0444)
Neogenomics Laboratories
www.neogenomics.org
1-866-776-5907
Caris Life Sciences
www.carislifesciences.com/hematology
1-800-901-5177
CSI Laboratories
www.csilaboratories.com
(800) 459-1185
Genzyme Genetics
www.genzymegenetics.com
1-800-447-5816
Molecular Pathology Laboratory Network, Inc.
www.MPLNET.com
1-800-932-2943
Acupath Laboratories, Inc.
www.acupath.com
1-888-ACUPATH (228-2784)
CPA Lab
www.cpalab.com
502-897-9594
Mayo Medical Laboratories
www.mayomedicallaboratories.com
United States and Canada: 800.533.1710 International: 507.266.5700
University of Miami
Miller School of Medicine IML Laboratories
305-243-9159 /5795 /5807
Yellowstone Pathology Institute, Inc
www.yellowstonepathology.com1-888-400-6640
1. Borowitz MJ, Craig FE, DiGiuseppe JA, et al; for Clinical Cytometry Society. Cytometry Part B Clin Cytom. doi:10.1002/cyto.b.20525. 2. Brodsky RA. Blood. 2009;113:6522-6527.
PNH Facts:
35% of patients with PNH die within 5 years of diagnosis. Thrombosis is the leading cause of death.
Patients with PNH may report abdominal pain, exhaustion, dark urine, or erectile dysfunction.